Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2202982..2203850 Replicon chromosome
Accession NZ_CP027035
Organism Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag C1D11_RS10680 Protein ID WP_010886136.1
Coordinates 2202982..2203359 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag C1D11_RS10685 Protein ID WP_003409886.1
Coordinates 2203401..2203850 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1D11_RS10630 2198771..2199223 - 453 WP_010950636.1 lipoprotein -
C1D11_RS10635 2199287..2199688 + 402 WP_003409869.1 hypothetical protein -
C1D11_RS10640 2199681..2199863 - 183 WP_003409870.1 hypothetical protein -
C1D11_RS10645 2199977..2200327 - 351 WP_003409871.1 hypothetical protein -
C1D11_RS10650 2200338..2201240 - 903 WP_003409874.1 hypothetical protein -
C1D11_RS10655 2201261..2201452 - 192 WP_003409876.1 hypothetical protein -
C1D11_RS10660 2201453..2201749 - 297 WP_003409877.1 hypothetical protein -
C1D11_RS10665 2201989..2202204 + 216 WP_003409878.1 antitoxin -
C1D11_RS10670 2202201..2202512 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
C1D11_RS21595 2202486..2203007 - 522 WP_010950637.1 hypothetical protein -
C1D11_RS10680 2202982..2203359 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
C1D11_RS10685 2203401..2203850 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C1D11_RS10690 2203847..2204392 + 546 WP_003409891.1 SecB-like chaperone -
C1D11_RS10695 2204281..2204895 - 615 WP_003901296.1 hypothetical protein -
C1D11_RS10700 2204944..2205240 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
C1D11_RS10705 2205237..2205488 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
C1D11_RS10710 2205475..2205969 + 495 WP_003899099.1 hypothetical protein -
C1D11_RS10715 2206129..2206536 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
C1D11_RS10720 2206540..2206812 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
C1D11_RS10725 2206845..2208065 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T97081 WP_010886136.1 NZ_CP027035:2202982-2203359 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T97081 NZ_CP027035:2202982-2203359 [Mycobacterium tuberculosis variant bovis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT97081 WP_003409886.1 NZ_CP027035:2203401-2203850 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT97081 NZ_CP027035:2203401-2203850 [Mycobacterium tuberculosis variant bovis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References