Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 1764755..1765383 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WFA5 |
| Locus tag | Rv1561 | Protein ID | NP_216077.1 |
| Coordinates | 1764979..1765383 (+) | Length | 135 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WLU3 |
| Locus tag | Rv1560 | Protein ID | NP_216076.1 |
| Coordinates | 1764755..1764973 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1554 | 1760175..1760555 | + | 381 | NP_216070.1 | fumarate reductase membrane anchor subunit | - |
| Rv1555 | 1760552..1760929 | + | 378 | NP_216071.1 | fumarate reductase membrane anchor subunit | - |
| Rv1556 | 1760997..1761605 | + | 609 | NP_216072.1 | HTH-type transcriptional regulator | - |
| Rv1557 | 1761744..1762937 | + | 1194 | NP_216073.1 | transmembrane transport protein MmpL6 | - |
| Rv1558 | 1762947..1763393 | + | 447 | NP_216074.1 | hypothetical protein | - |
| Rv1559 | 1763428..1764717 | + | 1290 | NP_216075.1 | threonine dehydratase IlvA | - |
| Rv1560 | 1764755..1764973 | + | 219 | NP_216076.1 | antitoxin VapB11 | Antitoxin |
| Rv1561 | 1764979..1765383 | + | 405 | NP_216077.1 | ribonuclease VapC11 | Toxin |
| Rv1562c | 1765400..1767142 | - | 1743 | YP_177819.1 | malto-oligosyltrehalose trehalohydrolase | - |
| Rv1563c | 1767135..1769432 | - | 2298 | YP_177820.1 | maltooligosyl trehalose synthase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 135 a.a. Molecular weight: 14650.76 Da Isoelectric Point: 5.6587
>T97 NP_216077.1 NC_000962:1764979-1765383 [Mycobacterium tuberculosis H37Rv]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR
Download Length: 405 bp
>T97 NC_000962:1764979-1765383 [Mycobacterium tuberculosis H37Rv]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8209.32 Da Isoelectric Point: 4.5600
>AT97 NP_216076.1 NC_000962:1764755-1764973 [Mycobacterium tuberculosis H37Rv]
VYRWCMSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD
VYRWCMSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD
Download Length: 219 bp
>AT97 NC_000962:1764755-1764973 [Mycobacterium tuberculosis H37Rv]
GTGTATCGTTGGTGTATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCT
GACCACCAAGAGGGCGGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGC
TGGAAGGCGTCGGCTGGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA
GTGTATCGTTGGTGTATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCT
GACCACCAAGAGGGCGGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGC
TGGAAGGCGTCGGCTGGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T107 | Mycobacterium tuberculosis H37Rv |
37.008 |
96.212 |
0.356 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT107 | Mycobacterium tuberculosis H37Rv |
54.412 |
94.444 |
0.514 |
| AT83 | Mycobacterium tuberculosis H37Rv |
52.941 |
66.667 |
0.353 |
Multiple sequence alignment
References
(1) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]
(2) Amar Deep et al. (2018) Structural, functional and biological insights into the role of Mycobacterium tuberculosis VapBC11 toxin-antitoxin system: targeting a tRNase to tackle mycobacterial adaptation. Nucleic Acids Research 46(21):11639-11655. [PubMed:30329074]