Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/RHH-PIN
Location 752984..753617 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB5
Locus tag Rv0656c Protein ID NP_215170.1
Coordinates 752984..753367 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ57
Locus tag Rv0657c Protein ID NP_215171.1
Coordinates 753462..753617 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv0651 748276..748812 + 537 NP_215165.1 50S ribosomal protein L10 -
Rv0652 748849..749241 + 393 NP_215166.1 50S ribosomal protein L7/L12 -
Rv0653c 749234..749929 - 696 NP_215167.1 transcriptional regulator -
Rv0654 750000..751505 + 1506 NP_215168.1 carotenoid cleavage oxygenase -
Rv0655 751517..752596 + 1080 NP_215169.1 ABC transporter ATP-binding protein -
Rv0656c 752984..753367 - 384 NP_215170.1 ribonuclease VapC6 Toxin
Rv0657c 753462..753617 - 156 NP_215171.1 antitoxin VapB6 Antitoxin
Rv0658c 753693..754409 - 717 NP_215172.1 integral membrane protein -
Rv0659c 754685..754993 - 309 NP_215173.1 toxin MazF2 -
Rv0660c 754980..755225 - 246 NP_215174.1 antitoxin MazE2 -
Rv0661c 755335..755772 - 438 NP_215175.1 ribonuclease VapC7 -
Rv0662c 755769..756023 - 255 NP_215176.2 antitoxin VapB7 -
Rv0663 756137..758500 + 2364 NP_215177.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T83 NP_215170.1 NC_000962:c753367-752984 [Mycobacterium tuberculosis H37Rv]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T83 NC_000962:c753367-752984 [Mycobacterium tuberculosis H37Rv]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT83 NP_215171.1 NC_000962:c753617-753462 [Mycobacterium tuberculosis H37Rv]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT83 NC_000962:c753617-753462 [Mycobacterium tuberculosis H37Rv]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value
AT107 Mycobacterium tuberculosis H37Rv

45.833

94.118

0.431
AT97 Mycobacterium tuberculosis H37Rv

52.941

66.667

0.353

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB P9WFB5

Antitoxin

Source ID Structure
AlphaFold DB P9WJ57

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature