Detailed information of TA system TA096980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 482310..482935
• Replicon: chromosome
• Accession: NZ_CP026985
• Organism: Pectobacterium parmentieri strain IFB5619

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3FNM7
• Locus tag: C5E25_RS02420
• Protein ID: WP_033070820.1
• Coordinates: 482310..482615 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5E25_RS02425
• Protein ID: WP_103808699.1
• Coordinates: 482612..482935 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E25_RS02390	477909..478643	+	735	WP_103808698.1	type I-E CRISPR-associated protein Cas5/CasD	-
C5E25_RS02395	478643..479290	+	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
C5E25_RS02400	479318..480235	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
C5E25_RS02405	480236..480529	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
C5E25_RS02410	481453..481824	-	372	WP_103808702.1	helix-turn-helix domain-containing protein	-
C5E25_RS02415	481898..482158	-	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C5E25_RS02420	482310..482615	+	306	WP_033070820.1	type II toxin-antitoxin system HigB family toxin	Toxin
C5E25_RS02425	482612..482935	+	324	WP_103808699.1	helix-turn-helix domain-containing protein	Antitoxin
C5E25_RS02430	484098..484556	+	459	WP_012822197.1	hypothetical protein	-
C5E25_RS02435	484637..485989	-	1353	WP_103808643.1	aspartate aminotransferase family protein	-
C5E25_RS02440	486020..487291	-	1272	WP_103808673.1	MFS transporter	-
C5E25_RS02445	487446..487892	+	447	WP_012822200.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12010.78 Da
• Isoelectric Point: 10.0386

>T96980 WP_033070820.1 NZ_CP026985:482310-482615 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T96980 NZ_CP026985:482310-482615 [Pectobacterium parmentieri]
GTGTTCTCCCGTGCACCTTTTGACACTGCAATCGCCCAATTCCCGAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11655.44 Da
• Isoelectric Point: 5.0877

>AT96980 WP_103808699.1 NZ_CP026985:482612-482935 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDAPEFVAFNERIKALPAGIALLRILMDQHGLTQSDFEAEIGKKSLVSRIL
NGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 324 bp

>AT96980 NZ_CP026985:482612-482935 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTTGTAA
AGATTATGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATCGCTTTGCTGCGCA
TACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGTATCCCGAATTCTA
AATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGAGTGCTTTTGTTGA
TTAG

Structures

Toxin

• AlphaFold DB: A0A8B3FNM7