Detailed information of TA system TA096968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4413575..4414293
• Replicon: chromosome
• Accession: NZ_CP026982
• Organism: Pectobacterium parmentieri strain IFB5486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3FNM7
• Locus tag: C5E22_RS20565
• Protein ID: WP_033070820.1
• Coordinates: 4413988..4414293 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B3FM67
• Locus tag: C5E22_RS20560
• Protein ID: WP_033070769.1
• Coordinates: 4413575..4413991 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E22_RS20540	4408622..4409068	-	447	WP_012822200.1	helix-turn-helix transcriptional regulator	-
C5E22_RS20545	4409223..4410494	+	1272	WP_033070821.1	MFS transporter	-
C5E22_RS20550	4410525..4411877	+	1353	WP_033070770.1	aspartate aminotransferase family protein	-
C5E22_RS20555	4411958..4412416	-	459	WP_012822197.1	hypothetical protein	-
C5E22_RS20560	4413575..4413991	-	417	WP_033070769.1	helix-turn-helix domain-containing protein	Antitoxin
C5E22_RS20565	4413988..4414293	-	306	WP_033070820.1	type II toxin-antitoxin system HigB family toxin	Toxin
C5E22_RS20570	4414445..4414705	+	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C5E22_RS20575	4414779..4415150	+	372	WP_012822192.1	helix-turn-helix domain-containing protein	-
C5E22_RS20580	4416318..4416611	-	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
C5E22_RS20585	4416612..4417529	-	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
C5E22_RS20590	4417557..4418204	-	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
C5E22_RS20595	4418204..4418938	-	735	WP_012822189.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12010.78 Da
• Isoelectric Point: 10.0386

>T96968 WP_033070820.1 NZ_CP026982:c4414293-4413988 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T96968 NZ_CP026982:c4414293-4413988 [Pectobacterium parmentieri]
GTGTTCTCCCGGGCACCTTTTGACACTGCAATCGCCCAATTCCCTAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15233.53 Da
• Isoelectric Point: 4.3708

>AT96968 WP_033070769.1 NZ_CP026982:c4413991-4413575 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDALKLIEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 417 bp

>AT96968 NZ_CP026982:c4413991-4413575 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTTGTAA
AGATTATGAGGATGCGCTGAAACTCATTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
ATCCCGAATTCTAAATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTGTTGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B3FNM7

Antitoxin

• AlphaFold DB: A0A8B3FM67