Detailed information of TA system TA096963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3129644..3130311
• Replicon: chromosome
• Accession: NZ_CP026982
• Organism: Pectobacterium parmentieri strain IFB5486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3F7E4
• Locus tag: C5E22_RS14535
• Protein ID: WP_033071239.1
• Coordinates: 3129997..3130311 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5E22_RS14530
• Protein ID: WP_015841345.1
• Coordinates: 3129644..3129985 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E22_RS14485	3124907..3126022	+	1116	WP_033071248.1	phage major capsid protein, P2 family	-
C5E22_RS14490	3126026..3126685	+	660	WP_033071247.1	terminase endonuclease subunit	-
C5E22_RS14495	3126777..3127280	+	504	WP_033071246.1	head completion/stabilization protein	-
C5E22_RS14500	3127280..3127483	+	204	WP_033071245.1	tail protein X	-
C5E22_RS14505	3127474..3127695	+	222	WP_033071244.1	hypothetical protein	-
C5E22_RS14510	3127679..3128188	+	510	WP_033071243.1	lysozyme	-
C5E22_RS14515	3128185..3128628	+	444	WP_033071242.1	Rz-like lysis system protein LysB	-
C5E22_RS14520	3128718..3129173	+	456	WP_033071241.1	phage tail protein	-
C5E22_RS14525	3129166..3129615	+	450	WP_033071240.1	phage virion morphogenesis protein	-
C5E22_RS14530	3129644..3129985	-	342	WP_015841345.1	HigA family addiction module antidote protein	Antitoxin
C5E22_RS14535	3129997..3130311	-	315	WP_033071239.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5E22_RS14540	3130655..3131284	+	630	WP_033071238.1	ORF6N domain-containing protein	-
C5E22_RS14545	3131330..3132079	-	750	WP_127087869.1	hypothetical protein	-
C5E22_RS14550	3132186..3132821	+	636	WP_033071236.1	phage baseplate assembly protein V	-
C5E22_RS14555	3132818..3133162	+	345	WP_033071235.1	GPW/gp25 family protein	-
C5E22_RS14560	3133167..3134075	+	909	WP_033071234.1	baseplate assembly protein	-
C5E22_RS14565	3134068..3134679	+	612	WP_033071233.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12183.99 Da
• Isoelectric Point: 10.1205

>T96963 WP_033071239.1 NZ_CP026982:c3130311-3129997 [Pectobacterium parmentieri]
MQKMRSIHSFRDKWLEDFFMYGKSHKKIPADVVTSLSRKLDIINAAVSAKDLRSPPGNRYENLNPPLGEYSSIRVNIQYR
LIFKWVDGKAIDVYLDDHGYKNHK

Nucleotide

• Length: 315 bp

>T96963 NZ_CP026982:c3130311-3129997 [Pectobacterium parmentieri]
ATGCAAAAAATGCGCAGCATTCATAGCTTTCGGGATAAGTGGCTGGAAGATTTTTTTATGTATGGTAAGTCGCATAAAAA
GATACCTGCCGATGTGGTTACATCCTTGTCCCGCAAGCTGGATATCATCAACGCGGCGGTATCGGCAAAAGATCTGAGAT
CGCCACCGGGGAACCGATACGAGAATCTCAACCCACCATTGGGGGAATACTCATCCATCAGAGTGAATATCCAATATCGC
CTGATTTTTAAATGGGTTGATGGTAAAGCGATTGATGTTTATCTGGATGACCACGGTTACAAAAATCATAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12941.56 Da
• Isoelectric Point: 4.4346

>AT96963 WP_015841345.1 NZ_CP026982:c3129985-3129644 [Pectobacterium parmentieri]
MAQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTISALVNNNRKLTVDMAFRLAKAFDTSVDFWLNLQNSVDVW
EVENDARTQEEISRITTLENFLAQRDENVKDVA

Nucleotide

• Length: 342 bp

>AT96963 NZ_CP026982:c3129985-3129644 [Pectobacterium parmentieri]
ATGGCACAGGCACAACGCAAGCCGACATCCGTAGGCGATGTTCTGTTGTATGAATATCTTGAACCGACGGGGTTGAAGAT
TAATGACCTGGCGGAAATGCTGAACGTCCACCGCAATACGATCAGCGCATTGGTCAACAACAACCGTAAGCTAACGGTTG
ATATGGCTTTCCGGTTGGCGAAAGCGTTTGATACATCAGTTGATTTCTGGCTTAACCTGCAAAATAGTGTTGACGTATGG
GAAGTGGAAAATGACGCGCGCACTCAGGAAGAAATTAGCCGGATCACCACGCTGGAAAATTTTCTGGCGCAGCGCGATGA
AAATGTGAAAGACGTCGCATAA

Structures

Toxin

• AlphaFold DB: A0A8B3F7E4