Detailed information of TA system TA096952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 481481..482106
• Replicon: chromosome
• Accession: NZ_CP026981
• Organism: Pectobacterium parmentieri strain IFB5485

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3FNM7
• Locus tag: C5E21_RS02400
• Protein ID: WP_033070820.1
• Coordinates: 481481..481786 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5E21_RS02405
• Protein ID: WP_103808699.1
• Coordinates: 481783..482106 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E21_RS02370	477080..477814	+	735	WP_103808698.1	type I-E CRISPR-associated protein Cas5/CasD	-
C5E21_RS02375	477814..478461	+	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
C5E21_RS02380	478489..479406	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
C5E21_RS02385	479407..479700	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
C5E21_RS02390	480624..480995	-	372	WP_103808702.1	helix-turn-helix domain-containing protein	-
C5E21_RS02395	481069..481329	-	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C5E21_RS02400	481481..481786	+	306	WP_033070820.1	type II toxin-antitoxin system HigB family toxin	Toxin
C5E21_RS02405	481783..482106	+	324	WP_103808699.1	helix-turn-helix domain-containing protein	Antitoxin
C5E21_RS02410	483269..483727	+	459	WP_012822197.1	hypothetical protein	-
C5E21_RS02415	483808..485160	-	1353	WP_103808643.1	aspartate aminotransferase family protein	-
C5E21_RS02420	485191..486462	-	1272	WP_103808673.1	MFS transporter	-
C5E21_RS02425	486617..487063	+	447	WP_012822200.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12010.78 Da
• Isoelectric Point: 10.0386

>T96952 WP_033070820.1 NZ_CP026981:481481-481786 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T96952 NZ_CP026981:481481-481786 [Pectobacterium parmentieri]
GTGTTCTCCCGTGCACCTTTTGACACTGCAATCGCCCAATTCCCGAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11655.44 Da
• Isoelectric Point: 5.0877

>AT96952 WP_103808699.1 NZ_CP026981:481783-482106 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDAPEFVAFNERIKALPAGIALLRILMDQHGLTQSDFEAEIGKKSLVSRIL
NGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 324 bp

>AT96952 NZ_CP026981:481783-482106 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTTGTAA
AGATTATGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATCGCTTTGCTGCGCA
TACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGTATCCCGAATTCTA
AATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGAGTGCTTTTGTTGA
TTAG

Structures

Toxin

• AlphaFold DB: A0A8B3FNM7