Detailed information of TA system TA096937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 442522..443240
• Replicon: chromosome
• Accession: NZ_CP026979
• Organism: Pectobacterium parmentieri strain IFB5432

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3FNM7
• Locus tag: C5E19_RS02170
• Protein ID: WP_033070820.1
• Coordinates: 442522..442827 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5E19_RS02175
• Protein ID: WP_012822195.1
• Coordinates: 442824..443240 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E19_RS02140	438000..438734	+	735	WP_012822189.1	type I-E CRISPR-associated protein Cas5/CasD	-
C5E19_RS02145	438734..439381	+	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
C5E19_RS02150	439409..440326	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
C5E19_RS02155	440327..440620	+	294	WP_121264021.1	type I-E CRISPR-associated endoribonuclease Cas2	-
C5E19_RS02160	441665..442036	-	372	WP_014698583.1	helix-turn-helix domain-containing protein	-
C5E19_RS02165	442110..442370	-	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C5E19_RS02170	442522..442827	+	306	WP_033070820.1	type II toxin-antitoxin system HigB family toxin	Toxin
C5E19_RS02175	442824..443240	+	417	WP_012822195.1	helix-turn-helix domain-containing protein	Antitoxin
C5E19_RS02180	443976..444434	+	459	WP_012822197.1	hypothetical protein	-
C5E19_RS02185	444515..445867	-	1353	WP_012822198.1	aspartate aminotransferase family protein	-
C5E19_RS02190	445898..447169	-	1272	WP_121266622.1	MFS transporter	-
C5E19_RS02195	447324..447770	+	447	WP_012822200.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12010.78 Da
• Isoelectric Point: 10.0386

>T96937 WP_033070820.1 NZ_CP026979:442522-442827 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T96937 NZ_CP026979:442522-442827 [Pectobacterium parmentieri]
GTGTTCTCCCGTGCACCTTTTGACACTGCAATCGCCCAATTCCCGAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15274.52 Da
• Isoelectric Point: 4.4634

>AT96937 WP_012822195.1 NZ_CP026979:442824-443240 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSRKDYEDALKLTEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 417 bp

>AT96937 NZ_CP026979:442824-443240 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTCGTAA
AGATTATGAGGATGCGCTGAAACTCACTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
ATCCCGAATTCTAAATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTGTTGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B3FNM7