Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 460413..461131 Replicon chromosome
Accession NZ_CP026977
Organism Pectobacterium parmentieri strain IFB5408

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3FNM7
Locus tag C5E17_RS02220 Protein ID WP_033070820.1
Coordinates 460413..460718 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3FM67
Locus tag C5E17_RS02225 Protein ID WP_033070769.1
Coordinates 460715..461131 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5E17_RS02190 455768..456502 + 735 WP_012822189.1 type I-E CRISPR-associated protein Cas5/CasD -
C5E17_RS02195 456502..457149 + 648 WP_012822190.1 type I-E CRISPR-associated protein Cas6/Cse3/CasE -
C5E17_RS02200 457177..458094 + 918 WP_012822191.1 type I-E CRISPR-associated endonuclease Cas1 -
C5E17_RS02205 458095..458388 + 294 WP_005973730.1 type I-E CRISPR-associated endoribonuclease Cas2 -
C5E17_RS02210 459556..459927 - 372 WP_012822192.1 helix-turn-helix domain-containing protein -
C5E17_RS02215 460001..460261 - 261 WP_012822193.1 type II toxin-antitoxin system RelE/ParE family toxin -
C5E17_RS02220 460413..460718 + 306 WP_033070820.1 type II toxin-antitoxin system HigB family toxin Toxin
C5E17_RS02225 460715..461131 + 417 WP_033070769.1 helix-turn-helix domain-containing protein Antitoxin
C5E17_RS02230 462290..462748 + 459 WP_012822197.1 hypothetical protein -
C5E17_RS02235 462829..464181 - 1353 WP_033070770.1 aspartate aminotransferase family protein -
C5E17_RS02240 464212..465483 - 1272 WP_033070821.1 MFS transporter -
C5E17_RS02245 465638..466084 + 447 WP_012822200.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(18-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 12010.78 Da        Isoelectric Point: 10.0386

>T96929 WP_033070820.1 NZ_CP026977:460413-460718 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Download         Length: 306 bp

>T96929 NZ_CP026977:460413-460718 [Pectobacterium parmentieri]
GTGTTCTCCCGGGCACCTTTTGACACTGCAATCGCCCAATTCCCTAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15233.53 Da        Isoelectric Point: 4.3708

>AT96929 WP_033070769.1 NZ_CP026977:460715-461131 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDALKLIEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTVDHMSALAKRFDIPVSAFVD

Download         Length: 417 bp

>AT96929 NZ_CP026977:460715-461131 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTTGTAA
AGATTATGAGGATGCGCTGAAACTCATTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
ATCCCGAATTCTAAATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTGTTGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNM7

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3FM67

References