Detailed information of TA system TA096920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4459924..4460584
• Replicon: chromosome
• Accession: NZ_CP026975
• Organism: Enterobacter cloacae complex sp. strain FDAARGOS_77

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MC67_RS22270
• Protein ID: WP_100245677.1
• Coordinates: 4459924..4460277 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: MC67_RS22275
• Protein ID: WP_029741460.1
• Coordinates: 4460282..4460584 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC67_RS22250	4456066..4456983	+	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-
MC67_RS22255	4457080..4458030	+	951	WP_010432106.1	HTH-type transcriptional regulator Cbl	-
MC67_RS22260	4458074..4458682	-	609	WP_057061635.1	glutathione S-transferase family protein	-
MC67_RS22265	4458853..4459746	+	894	WP_047063523.1	LysR family transcriptional regulator	-
MC67_RS22270	4459924..4460277	+	354	WP_100245677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MC67_RS22275	4460282..4460584	+	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
MC67_RS22280	4460661..4461581	-	921	WP_045403136.1	LysR family transcriptional regulator	-
MC67_RS22285	4461723..4462112	+	390	WP_023312340.1	RidA family protein	-
MC67_RS22295	4462436..4464625	-	2190	WP_045403139.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13432.23 Da
• Isoelectric Point: 9.0310

>T96920 WP_100245677.1 NZ_CP026975:4459924-4460277 [Enterobacter cloacae complex sp.]
VSAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Nucleotide

• Length: 354 bp

>T96920 NZ_CP026975:4459924-4460277 [Enterobacter cloacae complex sp.]
GTGTCGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTTACTTCGCTTGACGATACCGAACGCGCCAGCGTACTTGC
AGCCCTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCCGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGAATACATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT96920 WP_029741460.1 NZ_CP026975:4460282-4460584 [Enterobacter cloacae complex sp.]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT96920 NZ_CP026975:4460282-4460584 [Enterobacter cloacae complex sp.]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCCCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTCGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79