Detailed information of TA system TA096764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4776957..4777559
• Replicon: chromosome
• Accession: NZ_CP026939
• Organism: Escherichia coli strain CFS3313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C5F74_RS22800
• Protein ID: WP_000897305.1
• Coordinates: 4777248..4777559 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5F74_RS22795
• Protein ID: WP_000356397.1
• Coordinates: 4776957..4777247 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F74_RS22770	4772882..4773784	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C5F74_RS22775	4773781..4774416	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C5F74_RS22780	4774413..4775342	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C5F74_RS22785	4775672..4775914	-	243	WP_001087409.1	hypothetical protein	-
C5F74_RS22790	4776134..4776352	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
C5F74_RS22795	4776957..4777247	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C5F74_RS22800	4777248..4777559	-	312	WP_000897305.1	hypothetical protein	Toxin
C5F74_RS22805	4777788..4778696	+	909	WP_001162704.1	alpha/beta hydrolase	-
C5F74_RS22810	4778760..4779701	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C5F74_RS22815	4779746..4780183	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C5F74_RS22820	4780180..4781052	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C5F74_RS22825	4781046..4781645	-	600	WP_001295269.1	glucose-1-phosphatase	-
C5F74_RS22830	4781744..4782529	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T96764 WP_000897305.1 NZ_CP026939:c4777559-4777248 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T96764 NZ_CP026939:c4777559-4777248 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT96764 WP_000356397.1 NZ_CP026939:c4777247-4776957 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT96764 NZ_CP026939:c4777247-4776957 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7