96760

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3865050..3865591 Replicon chromosome
Accession NZ_CP026939
Organism Escherichia coli strain CFS3313

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C5F74_RS18640 Protein ID WP_000615976.1
Coordinates 3865313..3865591 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C5F74_RS18635 Protein ID WP_000729704.1
Coordinates 3865050..3865310 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F74_RS18610 3860833..3861618 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
C5F74_RS18615 3861563..3863302 + 1740 WP_001296890.1 flagellar biosynthesis protein FlhA -
C5F74_RS18620 3863407..3863685 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C5F74_RS18625 3863678..3864034 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
C5F74_RS18630 3864091..3864864 - 774 WP_000543895.1 C40 family peptidase -
C5F74_RS18635 3865050..3865310 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C5F74_RS18640 3865313..3865591 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C5F74_RS18645 3865747..3866487 + 741 WP_001225679.1 murein L,D-transpeptidase -
C5F74_RS18650 3866458..3867225 - 768 WP_000333380.1 class II glutamine amidotransferase -
C5F74_RS18655 3867431..3868009 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3863678..3881577 17899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T96760 WP_000615976.1 NZ_CP026939:3865313-3865591 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T96760 NZ_CP026939:3865313-3865591 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT96760 WP_000729704.1 NZ_CP026939:3865050-3865310 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT96760 NZ_CP026939:3865050-3865310 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References