Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1941690..1942192 Replicon chromosome
Accession NZ_CP026939
Organism Escherichia coli strain CFS3313

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag C5F74_RS09235 Protein ID WP_000767831.1
Coordinates 1941938..1942192 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C5F74_RS09230 Protein ID WP_001259255.1
Coordinates 1941690..1941941 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F74_RS09205 1936867..1937934 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C5F74_RS09210 1937934..1939004 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
C5F74_RS09215 1939001..1940305 - 1305 WP_001565639.1 histidinol dehydrogenase -
C5F74_RS09220 1940311..1941210 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C5F74_RS09225 1941356..1941406 - 51 WP_001364200.1 his operon leader peptide -
C5F74_RS09230 1941690..1941941 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C5F74_RS09235 1941938..1942192 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C5F74_RS09240 1942275..1943099 + 825 WP_000754737.1 SDR family oxidoreductase -
C5F74_RS09245 1943145..1944074 + 930 WP_000803351.1 LysR family transcriptional regulator -
C5F74_RS09250 1944289..1944351 + 63 WP_010723108.1 membrane protein YoeI -
C5F74_RS09255 1944341..1945699 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C5F74_RS09260 1945878..1946936 + 1059 WP_000492339.1 transport protein YeeE -
C5F74_RS09265 1946950..1947177 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T96746 WP_000767831.1 NZ_CP026939:1941938-1942192 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T96746 NZ_CP026939:1941938-1942192 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT96746 WP_001259255.1 NZ_CP026939:1941690-1941941 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96746 NZ_CP026939:1941690-1941941 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References