Detailed information of TA system TA096734

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23945..24199
• Replicon: plasmid pCFS3292-2
• Accession: NZ_CP026937
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: C5F72_RS26320
• Protein ID: WP_001351576.1
• Coordinates: 23945..24151 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	24138..24199 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS26290	19970..20371	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
C5F72_RS26295	20304..20561	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
C5F72_RS26300	20654..21307	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
C5F72_RS26305	22248..23105	-	858	WP_061583826.1	incFII family plasmid replication initiator RepA	-
C5F72_RS26310	23098..23172	-	75	WP_061357229.1	RepA leader peptide Tap	-
C5F72_RS26315	23404..23661	-	258	WP_001541895.1	replication regulatory protein RepA	-
C5F72_RS26320	23945..24151	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	24138..24199	+	62	NuclAT_0	-	Antitoxin
-	24138..24199	+	62	NuclAT_0	-	Antitoxin
-	24138..24199	+	62	NuclAT_0	-	Antitoxin
-	24138..24199	+	62	NuclAT_0	-	Antitoxin
C5F72_RS26325	24541..24753	-	213	WP_001541896.1	ANR family transcriptional regulator	-
C5F72_RS26330	24888..25448	-	561	WP_001541898.1	fertility inhibition protein FinO	-
C5F72_RS26335	25503..26249	-	747	WP_001541899.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia / aac(3)-IIa / blaTEM-1A	-	1..131954	131954
	-	flank	IS/Tn	-	-	18908..19285	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T96734 WP_001351576.1 NZ_CP026937:c24151-23945 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T96734 NZ_CP026937:c24151-23945 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT96734 NZ_CP026937:24138-24199 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin