Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4013315..4014009 Replicon chromosome
Accession NZ_CP026935
Organism Escherichia coli strain CFS3292

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag C5F72_RS19760 Protein ID WP_001263489.1
Coordinates 4013315..4013713 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag C5F72_RS19765 Protein ID WP_000554758.1
Coordinates 4013716..4014009 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4008903..4008983 - 81 NuclAT_13 - -
- 4008903..4008983 - 81 NuclAT_13 - -
- 4008903..4008983 - 81 NuclAT_13 - -
- 4008903..4008983 - 81 NuclAT_13 - -
C5F72_RS19735 4009579..4010037 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C5F72_RS19740 4010298..4011755 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
C5F72_RS19745 4011812..4012333 - 522 Protein_3872 peptide chain release factor H -
C5F72_RS19750 4012329..4012535 - 207 Protein_3873 RtcB family protein -
C5F72_RS19755 4012853..4013305 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
C5F72_RS19760 4013315..4013713 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C5F72_RS19765 4013716..4014009 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C5F72_RS19770 4014061..4015116 - 1056 WP_001226164.1 DNA polymerase IV -
C5F72_RS19775 4015187..4015972 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
C5F72_RS19780 4015944..4017656 + 1713 Protein_3879 flagellar biosynthesis protein FlhA -
C5F72_RS19785 4017880..4018377 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T96727 WP_001263489.1 NZ_CP026935:c4013713-4013315 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T96727 NZ_CP026935:c4013713-4013315 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT96727 WP_000554758.1 NZ_CP026935:c4014009-4013716 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT96727 NZ_CP026935:c4014009-4013716 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References