Detailed information of TA system TA096718

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralR-agrB/-
• Location: 2173928..2174299
• Replicon: chromosome
• Accession: NZ_CP026935
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: Q8Z5Y6
• Locus tag: C5F72_RS10435
• Protein ID: WP_010989194.1
• Coordinates: 2174105..2174299 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	2173928..2174106 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS10395	2169370..2170026	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
C5F72_RS10400	2170128..2170358	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
C5F72_RS10405	2170497..2170871	+	375	WP_000168747.1	CopC domain-containing protein YobA	-
C5F72_RS10410	2170875..2171747	+	873	WP_000879289.1	copper homeostasis membrane protein CopD	-
C5F72_RS10415	2171760..2172101	+	342	WP_000976476.1	YebY family protein	-
C5F72_RS10420	2172494..2173570	-	1077	WP_001189085.1	phage integrase Arm DNA-binding domain-containing protein	-
C5F72_RS10425	2173536..2173817	-	282	WP_001311878.1	excisionase	-
C5F72_RS10430	2173924..2174112	-	189	WP_024165285.1	DUF1187 family protein	-
-	2173928..2174106	+	179	NuclAT_0	-	Antitoxin
-	2173928..2174106	+	179	NuclAT_0	-	Antitoxin
-	2173928..2174106	+	179	NuclAT_0	-	Antitoxin
-	2173928..2174106	+	179	NuclAT_0	-	Antitoxin
C5F72_RS10435	2174105..2174299	-	195	WP_010989194.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C5F72_RS10440	2174363..2175415	-	1053	WP_159375964.1	recombinase RecT	-
C5F72_RS10445	2175427..2178498	-	3072	WP_159375965.1	exodeoxyribonuclease VIII	-
C5F72_RS10450	2178598..2178873	-	276	WP_001417976.1	hypothetical protein	-
C5F72_RS10455	2178948..2179124	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2166627..2231780	65153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7077.01 Da
• Isoelectric Point: 9.2886

>T96718 WP_010989194.1 NZ_CP026935:c2174299-2174105 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T96718 NZ_CP026935:c2174299-2174105 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCAAAGTGTAACGGATGCGAATCCCGAACTGGCTATAGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT96718 NZ_CP026935:2173928-2174106 [Escherichia coli]
GAGGACAGAAGCTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCAGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A716R3V7

Antitoxin