Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1966674..1967176 Replicon chromosome
Accession NZ_CP026935
Organism Escherichia coli strain CFS3292

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C5F72_RS09415 Protein ID WP_000767829.1
Coordinates 1966922..1967176 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C5F72_RS09410 Protein ID WP_001259255.1
Coordinates 1966674..1966925 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F72_RS09385 1961852..1962919 - 1068 WP_001403117.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C5F72_RS09390 1962919..1963989 - 1071 WP_071345243.1 histidinol-phosphate transaminase -
C5F72_RS09395 1963986..1965290 - 1305 WP_001403116.1 histidinol dehydrogenase -
C5F72_RS09400 1965296..1966195 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C5F72_RS09405 1966341..1966391 - 51 WP_001364200.1 his operon leader peptide -
C5F72_RS09410 1966674..1966925 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C5F72_RS09415 1966922..1967176 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C5F72_RS09420 1967259..1968083 + 825 WP_000754737.1 SDR family oxidoreductase -
C5F72_RS09425 1968129..1969058 + 930 WP_063090662.1 LysR family transcriptional regulator -
C5F72_RS09430 1969273..1969335 + 63 WP_010723108.1 membrane protein YoeI -
C5F72_RS09435 1969325..1970683 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C5F72_RS09440 1970862..1971920 + 1059 WP_000492312.1 transport protein YeeE -
C5F72_RS09445 1971934..1972161 + 228 WP_000985278.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T96708 WP_000767829.1 NZ_CP026935:1966922-1967176 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T96708 NZ_CP026935:1966922-1967176 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT96708 WP_001259255.1 NZ_CP026935:1966674-1966925 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96708 NZ_CP026935:1966674-1966925 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References