Detailed information of TA system TA096695

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 99702..99935
• Replicon: plasmid pCFS3273-2
• Accession: NZ_CP026934
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C5F73_RS26500
• Protein ID: WP_001312861.1
• Coordinates: 99702..99860 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	99904..99935 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS26475	95075..95764	-	690	WP_000283383.1	conjugal transfer transcriptional regulator TraJ	-
C5F73_RS26480	95951..96334	-	384	WP_001151528.1	relaxosome protein TraM	-
C5F73_RS26485	96655..97257	+	603	WP_000243710.1	transglycosylase SLT domain-containing protein	-
C5F73_RS26490	97554..98375	-	822	WP_001234423.1	DUF945 domain-containing protein	-
C5F73_RS26495	98495..98692	-	198	WP_052318758.1	hypothetical protein	-
C5F73_RS26500	99702..99860	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	99904..99935	-	32	NuclAT_1	-	Antitoxin
-	99904..99935	-	32	NuclAT_1	-	Antitoxin
-	99904..99935	-	32	NuclAT_1	-	Antitoxin
-	99904..99935	-	32	NuclAT_1	-	Antitoxin
-	101377..101574	-	198	NuclAT_0	-	-
-	101377..101574	-	198	NuclAT_0	-	-
-	101377..101574	-	198	NuclAT_0	-	-
-	101377..101574	-	198	NuclAT_0	-	-
C5F73_RS26510	101386..101574	+	189	WP_001299721.1	hypothetical protein	-
C5F73_RS26515	101586..102305	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
C5F73_RS26520	102302..102736	-	435	WP_000845932.1	conjugation system SOS inhibitor PsiB	-
C5F73_RS26525	102791..104749	-	1959	Protein_122	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sul2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..145001	145001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T96695 WP_001312861.1 NZ_CP026934:c99860-99702 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T96695 NZ_CP026934:c99860-99702 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT96695 NZ_CP026934:c99935-99904 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin