Detailed information of TA system TA096668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1613982..1614676
• Replicon: chromosome
• Accession: NZ_CP026932
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: C5F73_RS07790
• Protein ID: WP_001263489.1
• Coordinates: 1614278..1614676 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C5F73_RS07785
• Protein ID: WP_000554758.1
• Coordinates: 1613982..1614275 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS07765	1609614..1610111	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
C5F73_RS07770	1610335..1612074	-	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
C5F73_RS07775	1612019..1612804	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
C5F73_RS07780	1612875..1613930	+	1056	WP_001226164.1	DNA polymerase IV	-
C5F73_RS07785	1613982..1614275	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C5F73_RS07790	1614278..1614676	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C5F73_RS07795	1614686..1615138	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
C5F73_RS07800	1615444..1615710	+	267	WP_001295202.1	hypothetical protein	-
C5F73_RS07805	1615622..1616179	+	558	WP_001352051.1	peptide chain release factor H	-
C5F73_RS07810	1616236..1617693	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
C5F73_RS07815	1617954..1618412	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1619008..1619088	+	81	NuclAT_10	-	-
-	1619008..1619088	+	81	NuclAT_10	-	-
-	1619008..1619088	+	81	NuclAT_10	-	-
-	1619008..1619088	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1600219..1630956	30737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T96668 WP_001263489.1 NZ_CP026932:1614278-1614676 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T96668 NZ_CP026932:1614278-1614676 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT96668 WP_000554758.1 NZ_CP026932:1613982-1614275 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT96668 NZ_CP026932:1613982-1614275 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3