Detailed information of TA system TA096647

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34003..34257
• Replicon: plasmid pCFS3246-1
• Accession: NZ_CP026930
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: C4962_RS23345
• Protein ID: WP_001367749.1
• Coordinates: 34003..34152 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34196..34257 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4962_RS23320	30130..30450	-	321	Protein_36	transposase	-
C4962_RS23325	30664..31422	+	759	WP_001257942.1	substrate-binding domain-containing protein	-
C4962_RS23330	32301..33158	-	858	WP_000130970.1	incFII family plasmid replication initiator RepA	-
C4962_RS23335	33151..33225	-	75	WP_001375168.1	RepA leader peptide Tap	-
C4962_RS23340	33471..33719	-	249	WP_000083838.1	replication regulatory protein RepA	-
C4962_RS23345	34003..34152	-	150	WP_001367749.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	34196..34257	+	62	NuclAT_1	-	Antitoxin
-	34196..34257	+	62	NuclAT_1	-	Antitoxin
-	34196..34257	+	62	NuclAT_1	-	Antitoxin
-	34196..34257	+	62	NuclAT_1	-	Antitoxin
C4962_RS24445	34516..34758	-	243	WP_000504693.1	hypothetical protein	-
C4962_RS23350	34837..35534	+	698	Protein_43	IS1-like element IS1A family transposase	-
C4962_RS23355	35759..36436	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
C4962_RS23360	36436..36783	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
C4962_RS23365	36803..38374	+	1572	WP_000381395.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(3'')-Ib / aph(6)-Id	estIa / paa	1..129225	129225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T96647 WP_001367749.1 NZ_CP026930:c34152-34003 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T96647 NZ_CP026930:c34152-34003 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT96647 NZ_CP026930:34196-34257 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin