Detailed information of TA system TA096642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3773558..3774252
• Replicon: chromosome
• Accession: NZ_CP026929
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: C4962_RS18295
• Protein ID: WP_001263489.1
• Coordinates: 3773558..3773956 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C4962_RS18300
• Protein ID: WP_000554758.1
• Coordinates: 3773959..3774252 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3769146..3769226	-	81	NuclAT_13	-	-
-	3769146..3769226	-	81	NuclAT_13	-	-
-	3769146..3769226	-	81	NuclAT_13	-	-
-	3769146..3769226	-	81	NuclAT_13	-	-
C4962_RS18270	3769822..3770280	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C4962_RS18275	3770541..3771998	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
C4962_RS18280	3772055..3772612	-	558	WP_001352051.1	peptide chain release factor H	-
C4962_RS18285	3772524..3772790	-	267	WP_001295202.1	hypothetical protein	-
C4962_RS18290	3773096..3773548	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
C4962_RS18295	3773558..3773956	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C4962_RS18300	3773959..3774252	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C4962_RS18305	3774304..3775359	-	1056	WP_001226164.1	DNA polymerase IV	-
C4962_RS18310	3775430..3776215	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
C4962_RS18315	3776160..3777899	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
C4962_RS18320	3778123..3778620	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3773558..3789614	16056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T96642 WP_001263489.1 NZ_CP026929:c3773956-3773558 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T96642 NZ_CP026929:c3773956-3773558 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT96642 WP_000554758.1 NZ_CP026929:c3774252-3773959 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT96642 NZ_CP026929:c3774252-3773959 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3