Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1876750..1877252 Replicon chromosome
Accession NZ_CP026929
Organism Escherichia coli strain CFS3246

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C4962_RS08905 Protein ID WP_000767829.1
Coordinates 1876998..1877252 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A6N8RC01
Locus tag C4962_RS08900 Protein ID WP_001259251.1
Coordinates 1876750..1877001 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4962_RS08875 1871927..1872994 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C4962_RS08880 1872994..1874064 - 1071 WP_032082632.1 histidinol-phosphate transaminase -
C4962_RS08885 1874061..1875365 - 1305 WP_032082631.1 histidinol dehydrogenase -
C4962_RS08890 1875371..1876270 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C4962_RS08895 1876416..1876466 - 51 WP_001364200.1 his operon leader peptide -
C4962_RS08900 1876750..1877001 + 252 WP_001259251.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C4962_RS08905 1876998..1877252 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C4962_RS08910 1877335..1878159 + 825 WP_000754737.1 SDR family oxidoreductase -
C4962_RS08915 1878205..1879134 + 930 WP_000803351.1 LysR family transcriptional regulator -
C4962_RS08920 1879349..1879411 + 63 WP_010723108.1 membrane protein YoeI -
C4962_RS08925 1879401..1880759 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C4962_RS08930 1880938..1881996 + 1059 WP_000492340.1 transport protein YeeE -
C4962_RS08935 1882010..1882237 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T96627 WP_000767829.1 NZ_CP026929:1876998-1877252 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T96627 NZ_CP026929:1876998-1877252 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9293.56 Da        Isoelectric Point: 4.7815

>AT96627 WP_001259251.1 NZ_CP026929:1876750-1877001 [Escherichia coli]
MRTISYSEARQNLSATMMKAVDDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96627 NZ_CP026929:1876750-1877001 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGATGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8RC01

References