Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2344736..2345277 Replicon chromosome
Accession NZ_CP026878
Organism Shigella dysenteriae strain 07-3308

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag C1P62_RS13020 Protein ID WP_000615980.1
Coordinates 2344999..2345277 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag C1P62_RS13015 Protein ID WP_000729709.1
Coordinates 2344736..2344996 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P62_RS12975 2339997..2340692 + 696 Protein_2461 IS1-like element IS1N family transposase -
C1P62_RS12980 2340706..2340918 + 213 Protein_2462 helix-turn-helix transcriptional regulator -
C1P62_RS12990 2341207..2341904 - 698 Protein_2463 IS1 family transposase -
C1P62_RS12995 2341959..2342303 - 345 Protein_2464 fimbria/pilus periplasmic chaperone -
C1P62_RS13000 2342534..2343227 - 694 Protein_2465 IS1 family transposase -
C1P62_RS13005 2343284..2343601 - 318 Protein_2466 transposase -
C1P62_RS13010 2343777..2344526 - 750 WP_011378552.1 C40 family peptidase -
C1P62_RS13015 2344736..2344996 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1P62_RS13020 2344999..2345277 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1P62_RS13025 2345433..2346173 + 741 WP_001225679.1 murein L,D-transpeptidase -
C1P62_RS13030 2346144..2346911 - 768 WP_000333379.1 class II glutamine amidotransferase -
C1P62_RS13035 2347117..2347695 - 579 WP_005020694.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimC / gmhA/lpcA 2338948..2364334 25386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T96523 WP_000615980.1 NZ_CP026878:2344999-2345277 [Shigella dysenteriae]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T96523 NZ_CP026878:2344999-2345277 [Shigella dysenteriae]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAGG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT96523 WP_000729709.1 NZ_CP026878:2344736-2344996 [Shigella dysenteriae]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT96523 NZ_CP026878:2344736-2344996 [Shigella dysenteriae]
ATGGCTGCAAATGCATTTGTTCGTGCCCGAATCGATGAGGATTTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References