Detailed information of TA system TA096512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 386038..386665
• Replicon: chromosome
• Accession: NZ_CP026878
• Organism: Shigella dysenteriae strain 07-3308

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A828BKW8
• Locus tag: C1P62_RS02015
• Protein ID: WP_024258354.1
• Coordinates: 386336..386665 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P62_RS02010
• Protein ID: WP_000147081.1
• Coordinates: 386038..386346 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P62_RS01975	381104..381664	+	561	WP_001215754.1	DUF1175 domain-containing protein	-
C1P62_RS01980	381722..382726	-	1005	Protein_385	tyrosine-type recombinase/integrase	-
C1P62_RS01985	382860..383404	+	545	Protein_386	transposase	-
C1P62_RS01995	384671..385360	+	690	Protein_388	IS3-like element IS600 family transposase	-
C1P62_RS02000	385413..385823	+	411	WP_000814621.1	recombination protein NinB	-
C1P62_RS02005	385820..386041	+	222	WP_001254231.1	NinE family protein	-
C1P62_RS02010	386038..386346	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P62_RS02015	386336..386665	-	330	WP_024258354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P62_RS02020	386986..387663	+	678	WP_000957540.1	phage antirepressor Ant	-
C1P62_RS02025	387738..388460	+	723	WP_001004003.1	DNA-binding protein	-
C1P62_RS02030	388498..388638	+	141	Protein_395	HNH endonuclease	-
C1P62_RS02040	388695..389389	+	695	WP_134801589.1	IS1 family transposase	-
C1P62_RS02045	389405..389725	-	321	Protein_397	ABC transporter substrate-binding protein ArtJ	-
C1P62_RS02050	390016..390684	-	669	WP_000464490.1	arginine ABC transporter permease ArtM	-
C1P62_RS02055	390684..391400	-	717	WP_001001693.1	arginine ABC transporter permease ArtQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	382860..396554	13694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12837.87 Da
• Isoelectric Point: 9.9966

>T96512 WP_024258354.1 NZ_CP026878:c386665-386336 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T96512 NZ_CP026878:c386665-386336 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT96512 WP_000147081.1 NZ_CP026878:c386346-386038 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT96512 NZ_CP026878:c386346-386038 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A828BKW8

Antitoxin

• AlphaFold DB: A0A4P7TJC1