Detailed information of TA system TA096504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4347906..4348508
• Replicon: chromosome
• Accession: NZ_CP026853
• Organism: Escherichia coli strain MS7163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MS7163_RS22725
• Protein ID: WP_000897302.1
• Coordinates: 4347906..4348217 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS7163_RS22730
• Protein ID: WP_000356397.1
• Coordinates: 4348218..4348508 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7163_RS22700	4343820..4344419	+	600	WP_001314324.1	glucose-1-phosphatase	-
MS7163_RS22705	4344413..4345285	+	873	WP_000920753.1	virulence factor BrkB family protein	-
MS7163_RS22710	4345282..4345719	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MS7163_RS22715	4345764..4346705	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MS7163_RS22720	4346769..4347677	-	909	WP_001331553.1	alpha/beta hydrolase	-
MS7163_RS22725	4347906..4348217	+	312	WP_000897302.1	hypothetical protein	Toxin
MS7163_RS22730	4348218..4348508	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MS7163_RS22740	4348867..4349145	+	279	WP_001296612.1	hypothetical protein	-
MS7163_RS22750	4349541..4349759	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
MS7163_RS22755	4349975..4350904	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MS7163_RS22760	4350901..4351536	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MS7163_RS22765	4351533..4352435	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T96504 WP_000897302.1 NZ_CP026853:4347906-4348217 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T96504 NZ_CP026853:4347906-4348217 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT96504 WP_000356397.1 NZ_CP026853:4348218-4348508 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT96504 NZ_CP026853:4348218-4348508 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416