Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2128958..2129460 Replicon chromosome
Accession NZ_CP026853
Organism Escherichia coli strain MS7163

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MS7163_RS11405 Protein ID WP_000767829.1
Coordinates 2128958..2129212 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MS7163_RS11410 Protein ID WP_001259255.1
Coordinates 2129209..2129460 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS7163_RS11375 2123973..2124200 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
MS7163_RS11380 2124214..2125272 - 1059 WP_000492340.1 transport protein YeeE -
MS7163_RS11385 2125451..2126809 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MS7163_RS11390 2126799..2126861 - 63 WP_010723108.1 membrane protein YoeI -
MS7163_RS11395 2127076..2128005 - 930 WP_000803341.1 LysR family transcriptional regulator -
MS7163_RS11400 2128051..2128875 - 825 WP_000754755.1 SDR family oxidoreductase -
MS7163_RS11405 2128958..2129212 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MS7163_RS11410 2129209..2129460 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MS7163_RS11415 2129744..2129794 + 51 WP_001364200.1 his operon leader peptide -
MS7163_RS11425 2129940..2130839 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MS7163_RS11430 2130845..2132149 + 1305 WP_001514311.1 histidinol dehydrogenase -
MS7163_RS11435 2132146..2133216 + 1071 WP_000108976.1 histidinol-phosphate transaminase -
MS7163_RS11440 2133216..2134283 + 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T96495 WP_000767829.1 NZ_CP026853:c2129212-2128958 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T96495 NZ_CP026853:c2129212-2128958 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT96495 WP_001259255.1 NZ_CP026853:c2129460-2129209 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96495 NZ_CP026853:c2129460-2129209 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References