Detailed information of TA system TA096480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 512640..513319
• Replicon: chromosome
• Accession: NZ_CP026853
• Organism: Escherichia coli strain MS7163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0VBP6
• Locus tag: MS7163_RS02540
• Protein ID: WP_000057524.1
• Coordinates: 512640..512942 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: MS7163_RS02545
• Protein ID: WP_000806442.1
• Coordinates: 512978..513319 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7163_RS02510	508016..509668	+	1653	WP_000771762.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
MS7163_RS02515	509706..510209	-	504	WP_000667000.1	hypothetical protein	-
MS7163_RS02520	510206..511006	-	801	WP_000439796.1	hypothetical protein	-
MS7163_RS02525	511030..511509	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
MS7163_RS02535	511713..512507	-	795	WP_000365177.1	TraB/GumN family protein	-
MS7163_RS02540	512640..512942	+	303	WP_000057524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS7163_RS02545	512978..513319	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
MS7163_RS02550	513377..515881	-	2505	WP_000083945.1	copper-exporting P-type ATPase CopA	-
MS7163_RS02555	516143..517075	+	933	WP_000883052.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	509706..521920	12214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11839.47 Da
• Isoelectric Point: 10.2237

>T96480 WP_000057524.1 NZ_CP026853:512640-512942 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T96480 NZ_CP026853:512640-512942 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT96480 WP_000806442.1 NZ_CP026853:512978-513319 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT96480 NZ_CP026853:512978-513319 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: V0VBP6

Antitoxin

• AlphaFold DB: S1QAY3