Detailed information of TA system TA096478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 283705..284399
• Replicon: chromosome
• Accession: NZ_CP026853
• Organism: Escherichia coli strain MS7163

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MS7163_RS01360
• Protein ID: WP_001263500.1
• Coordinates: 284001..284399 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MS7163_RS01355
• Protein ID: WP_000554758.1
• Coordinates: 283705..283998 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7163_RS01335	279241..279738	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
MS7163_RS28065	279821..279979	-	159	WP_014639450.1	hypothetical protein	-
MS7163_RS01340	280058..281770	-	1713	Protein_252	flagellar biosynthesis protein FlhA	-
MS7163_RS01345	281742..282527	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
MS7163_RS01350	282598..283653	+	1056	WP_001226168.1	DNA polymerase IV	-
MS7163_RS01355	283705..283998	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MS7163_RS01360	284001..284399	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MS7163_RS01365	284409..284861	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
MS7163_RS01370	285051..286190	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
MS7163_RS01375	286187..286801	+	615	WP_000602123.1	peptide chain release factor H	-
MS7163_RS01380	286858..288315	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MS7163_RS01385	288576..289034	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	264180..284861	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T96478 WP_001263500.1 NZ_CP026853:284001-284399 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T96478 NZ_CP026853:284001-284399 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT96478 WP_000554758.1 NZ_CP026853:283705-283998 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT96478 NZ_CP026853:283705-283998 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3