Detailed information of TA system TA096466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2775867..2776369
• Replicon: chromosome
• Accession: NZ_CP026850
• Organism: Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: AM451_RS13575
• Protein ID: WP_047050704.1
• Coordinates: 2776115..2776369 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: AM451_RS13570
• Protein ID: WP_047050702.1
• Coordinates: 2775867..2776118 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM451_RS13545 (AM451_13550)	2771046..2771240	+	195	WP_000644465.1	hypothetical protein	-
AM451_RS13550 (AM451_13555)	2771264..2771449	+	186	WP_001196016.1	hypothetical protein	-
AM451_RS13555 (AM451_13560)	2771517..2773613	+	2097	WP_047050699.1	peptidase domain-containing ABC transporter	-
AM451_RS13560 (AM451_13565)	2773610..2774359	+	750	WP_047050700.1	hypothetical protein	-
AM451_RS13565 (AM451_13570)	2774366..2775616	+	1251	WP_047050701.1	HlyD family efflux transporter periplasmic adaptor subunit	-
AM451_RS13570 (AM451_13575)	2775867..2776118	+	252	WP_047050702.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AM451_RS13575 (AM451_13580)	2776115..2776369	+	255	WP_047050704.1	Txe/YoeB family addiction module toxin	Toxin
AM451_RS24280	2776445..2776600	+	156	WP_154815872.1	hypothetical protein	-
AM451_RS13580 (AM451_13585)	2776731..2777819	-	1089	WP_047050706.1	AAA family ATPase	-
AM451_RS13585 (AM451_13590)	2777966..2778925	-	960	WP_047050707.1	DUF523 and DUF1722 domain-containing protein	-
AM451_RS13590 (AM451_13595)	2779028..2779438	-	411	Protein_2592	hydroxyisourate hydrolase	-
AM451_RS13595 (AM451_13600)	2779538..2780263	-	726	WP_017383646.1	MerR family transcriptional regulator	-
AM451_RS13600 (AM451_13605)	2780381..2780905	+	525	WP_047050709.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10117.66 Da
• Isoelectric Point: 7.8747

>T96466 WP_047050704.1 NZ_CP026850:2776115-2776369 [Enterobacter cloacae complex sp.]
VKITWSEEAWEDYLYWQETDKRMVRKINELIKISCRTPFEGIGKPEPLKHNLAGFWSRRITSEHRMVYAITEEAILIAAC
RYHY

Nucleotide

• Length: 255 bp

>T96466 NZ_CP026850:2776115-2776369 [Enterobacter cloacae complex sp.]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAGGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9343.57 Da
• Isoelectric Point: 4.7854

>AT96466 WP_047050702.1 NZ_CP026850:2775867-2776118 [Enterobacter cloacae complex sp.]
MRVISYSEARQNLSATMVQTTEDRAPVLITRQNGESCVLMSLEEYNSLEETAYLMRSPANAKRLMNSIESLKSGSGKERD
IIE

Nucleotide

• Length: 252 bp

>AT96466 NZ_CP026850:2775867-2776118 [Enterobacter cloacae complex sp.]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACCATGGTTCAGACGACGGAAGACCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTAGCGGGAAGGAAAGGGAC
ATTATTGAGTGA