Detailed information of TA system TA096461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 835570..836230
• Replicon: chromosome
• Accession: NZ_CP026850
• Organism: Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM451_RS04060
• Protein ID: WP_047053529.1
• Coordinates: 835877..836230 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q4Z7
• Locus tag: AM451_RS04055
• Protein ID: WP_017383313.1
• Coordinates: 835570..835872 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM451_RS04040 (AM451_04040)	831740..833065	+	1326	WP_047053533.1	SidA/IucD/PvdA family monooxygenase	-
AM451_RS04045 (AM451_04045)	833092..835281	+	2190	WP_047053531.1	TonB-dependent siderophore receptor	-
AM451_RS04055 (AM451_04055)	835570..835872	-	303	WP_017383313.1	XRE family transcriptional regulator	Antitoxin
AM451_RS04060 (AM451_04060)	835877..836230	-	354	WP_047053529.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM451_RS04065 (AM451_04065)	836421..837371	-	951	WP_003859532.1	HTH-type transcriptional regulator Cbl	-
AM451_RS04070 (AM451_04070)	837468..838385	-	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-
AM451_RS04080 (AM451_04080)	838874..839797	-	924	WP_047053526.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13522.40 Da
• Isoelectric Point: 10.1729

>T96461 WP_047053529.1 NZ_CP026850:c836230-835877 [Enterobacter cloacae complex sp.]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFNPNRTGI
VLCAGNKIGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T96461 NZ_CP026850:c836230-835877 [Enterobacter cloacae complex sp.]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTATTGCGGGAAAAGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAATTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCAATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGATCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11028.85 Da
• Isoelectric Point: 6.4727

>AT96461 WP_017383313.1 NZ_CP026850:c835872-835570 [Enterobacter cloacae complex sp.]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT96461 NZ_CP026850:c835872-835570 [Enterobacter cloacae complex sp.]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGGCGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTGCCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q4Z7