Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1912538..1913165 Replicon chromosome
Accession NZ_CP026846
Organism Shigella boydii strain 59-2708

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag C1P57_RS09445 Protein ID WP_024258354.1
Coordinates 1912836..1913165 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag C1P57_RS09440 Protein ID WP_000147081.1
Coordinates 1912538..1912846 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P57_RS09405 (1907823) 1907823..1908005 - 183 WP_134795662.1 transposase domain-containing protein -
C1P57_RS09410 (1908021) 1908021..1909177 + 1157 WP_086020934.1 IS3-like element IS600 family transposase -
C1P57_RS09415 (1909269) 1909269..1909856 + 588 Protein_1831 DNA replication protein -
C1P57_RS09420 (1909846) 1909846..1911282 + 1437 WP_134795663.1 DnaB-like helicase C-terminal domain-containing protein -
C1P57_RS09425 (1911359) 1911359..1911799 + 441 WP_000736913.1 recombination protein NinB -
C1P57_RS09430 (1911796) 1911796..1912323 + 528 WP_000153280.1 phage N-6-adenine-methyltransferase -
C1P57_RS09435 (1912320) 1912320..1912496 + 177 WP_078167352.1 NinE family protein -
C1P57_RS09440 (1912538) 1912538..1912846 - 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
C1P57_RS09445 (1912836) 1912836..1913165 - 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P57_RS09450 (1913372) 1913372..1913758 - 387 Protein_1838 TerL protein -
C1P57_RS09455 (1913761) 1913761..1914318 - 558 WP_078167353.1 terminase small subunit -
C1P57_RS09460 (1914420) 1914420..1914677 - 258 WP_078167354.1 hypothetical protein -
C1P57_RS09465 (1914674) 1914674..1915171 - 498 WP_078167355.1 KilA-N domain-containing protein -
C1P57_RS24710 (1915377) 1915377..1915844 - 468 WP_078167356.1 lysis protein -
C1P57_RS09475 (1915841) 1915841..1916338 - 498 WP_134795664.1 lysozyme RrrD -
C1P57_RS09480 (1916338) 1916338..1916553 - 216 WP_077123425.1 class II holin family protein -
C1P57_RS09495 (1917086) 1917086..1917460 - 375 Protein_1845 antitermination protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1907103..1927912 20809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T96436 WP_024258354.1 NZ_CP026846:c1913165-1912836 [Shigella boydii]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T96436 NZ_CP026846:c1913165-1912836 [Shigella boydii]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT96436 WP_000147081.1 NZ_CP026846:c1912846-1912538 [Shigella boydii]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT96436 NZ_CP026846:c1912846-1912538 [Shigella boydii]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References