Detailed information of TA system TA096431

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 1211933..1212346
• Replicon: chromosome
• Accession: NZ_CP026846
• Organism: Shigella boydii strain 59-2708

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: C1P57_RS05925
• Protein ID: WP_223200913.1
• Coordinates: 1211933..1212055 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	1212140..1212346 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P57_RS05895 (1207391)	1207391..1207738	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P57_RS05900 (1207758)	1207758..1208401	+	644	Protein_1151	IS66-like element ISSfl3 family transposase	-
C1P57_RS05910 (1209744)	1209744..1209878	+	135	Protein_1153	transposase	-
C1P57_RS05915 (1209948)	1209948..1211481	+	1534	WP_119181915.1	IS3 family transposase	-
C1P57_RS05925 (1211933)	1211933..1212055	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
C1P57_RS25060 (1212000)	1212000..1212152	-	153	Protein_1156	DUF5431 family protein	-
- (1212140)	1212140..1212346	-	207	NuclAT_5	-	Antitoxin
- (1212140)	1212140..1212346	-	207	NuclAT_5	-	Antitoxin
- (1212140)	1212140..1212346	-	207	NuclAT_5	-	Antitoxin
- (1212140)	1212140..1212346	-	207	NuclAT_5	-	Antitoxin
C1P57_RS25065 (1212532)	1212532..1212630	-	99	Protein_1157	IS66 family insertion sequence element accessory protein TnpB	-
C1P57_RS05940 (1212614)	1212614..1213555	-	942	Protein_1158	IS66 family transposase	-
C1P57_RS05945 (1213575)	1213575..1213921	-	347	Protein_1159	IS66 family insertion sequence element accessory protein TnpB	-
C1P57_RS05950 (1213921)	1213921..1214298	-	378	Protein_1160	IS66-like element accessory protein TnpA	-
C1P57_RS05955 (1214365)	1214365..1215711	+	1347	WP_000483769.1	IS4-like element IS4 family transposase	-
C1P57_RS05960 (1215902)	1215902..1216660	+	759	WP_001295562.1	(2E,6E)-farnesyl-diphosphate-specific ditrans,polycis-undecaprenyl-diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1205212..1215711	10499
	-	inside	IScluster/Tn	-	-	1205212..1215711	10499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T96431 WP_223200913.1 NZ_CP026846:c1212055-1211933 [Shigella boydii]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T96431 NZ_CP026846:c1212055-1211933 [Shigella boydii]
ATCGTGTGCTGCACATTGTTGATATTCACACTCCTGACCCGGAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 207 bp

>AT96431 NZ_CP026846:c1212346-1212140 [Shigella boydii]
TTACCGTGAACTGGCTGAACGACCAGATTATTTTCAAGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCTATGATG
TGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGACAAAAGCCCC
GTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin