Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 977075..977802 Replicon chromosome
Accession NZ_CP026840
Organism Shigella dysenteriae strain ATCC 9753

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C1P67_RS05200 Protein ID WP_000550189.1
Coordinates 977488..977802 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C1P67_RS05195 Protein ID WP_000560263.1
Coordinates 977075..977491 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P67_RS27005 (972436) 972436..974787 + 2352 Protein_987 alpha-glucosidase -
C1P67_RS05190 (975013) 975013..977030 + 2018 Protein_988 NADPH-dependent 2,4-dienoyl-CoA reductase -
C1P67_RS05195 (977075) 977075..977491 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C1P67_RS05200 (977488) 977488..977802 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C1P67_RS05205 (978087) 978087..979223 - 1137 WP_000018666.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C1P67_RS05210 (979308) 979308..979811 + 504 WP_005028882.1 M48 family metallopeptidase -
C1P67_RS05215 (979888) 979888..980571 + 684 WP_001183035.1 vancomycin high temperature exclusion protein -
C1P67_RS05220 (980650) 980650..981636 + 987 WP_005028890.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T96406 WP_000550189.1 NZ_CP026840:c977802-977488 [Shigella dysenteriae]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T96406 NZ_CP026840:c977802-977488 [Shigella dysenteriae]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT96406 WP_000560263.1 NZ_CP026840:c977491-977075 [Shigella dysenteriae]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT96406 NZ_CP026840:c977491-977075 [Shigella dysenteriae]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References