Detailed information of TA system TA096380

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 2538399..2538816
• Replicon: chromosome
• Accession: NZ_CP026836
• Organism: Shigella boydii strain ATCC 49812

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: C1P56_RS13400
• Protein ID: WP_223200913.1
• Coordinates: 2538694..2538816 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2538399..2538609 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P56_RS13360	2533866..2534723	-	858	WP_000922456.1	phosphatidate cytidylyltransferase	-
C1P56_RS13365	2534736..2535494	-	759	WP_004996908.1	(2E,6E)-farnesyl-diphosphate-specific ditrans,polycis-undecaprenyl-diphosphate synthase	-
C1P56_RS13370	2535685..2537031	-	1347	WP_004969946.1	IS4-like element IS4 family transposase	-
C1P56_RS13375	2537098..2537475	+	378	Protein_2603	IS66-like element accessory protein TnpA	-
C1P56_RS13380	2537475..2537822	+	348	WP_000622995.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P56_RS13385	2537886..2538128	+	243	Protein_2605	transposase	-
C1P56_RS28510	2538112..2538210	+	99	Protein_2606	IS66 family insertion sequence element accessory protein TnpB	-
-	2538396..2538609	+	214	NuclAT_8	-	-
-	2538396..2538609	+	214	NuclAT_8	-	-
-	2538396..2538609	+	214	NuclAT_8	-	-
-	2538396..2538609	+	214	NuclAT_8	-	-
-	2538399..2538609	-	211	-	-	Antitoxin
C1P56_RS28515	2538597..2538749	+	153	Protein_2607	DUF5431 family protein	-
C1P56_RS13400	2538694..2538816	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
C1P56_RS27870	2538909..2539073	+	165	WP_004989787.1	hypothetical protein	-
C1P56_RS13410	2540871..2541005	-	135	Protein_2611	transposase	-
C1P56_RS28520	2541005..2541109	-	105	WP_075288148.1	transposase domain-containing protein	-
C1P56_RS28525	2541131..2541625	-	495	Protein_2613	IS66 family transposase	-
C1P56_RS28530	2541565..2542464	-	900	WP_284542724.1	IS66 family transposase	-
C1P56_RS13420	2542631..2542978	-	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P56_RS13425	2542975..2543649	-	675	WP_001088287.1	IS66-like element accessory protein TnpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2535685..2545200	9515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T96380 WP_223200913.1 NZ_CP026836:2538694-2538816 [Shigella boydii]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T96380 NZ_CP026836:2538694-2538816 [Shigella boydii]
ATCGTGTGCTGCACATTGTTGATATTCACACTCCTGACCCGGAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 211 bp

>AT96380 NZ_CP026836:c2538609-2538399 [Shigella boydii]
GTGGACTAGACATGCAGAGGCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTTGTCCTTTGTCCTTCTGCCACACGA
CAGGATAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCT
GACCACACTCACTTTCCCTGAAAATAATCTGGTCGTTCAGCCAGTTCACGG

Structures

Antitoxin