Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2480512..2481053 Replicon chromosome
Accession NZ_CP026836
Organism Shigella boydii strain ATCC 49812

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag C1P56_RS13080 Protein ID WP_000615975.1
Coordinates 2480775..2481053 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C1P56_RS13075 Protein ID WP_000729704.1
Coordinates 2480512..2480772 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P56_RS13055 (2476187) 2476187..2476972 - 786 Protein_2546 putative lateral flagellar export/assembly protein LafU -
C1P56_RS13060 (2476944) 2476944..2478656 + 1713 Protein_2547 flagellar biosynthesis protein FlhA -
C1P56_RS13065 (2478880) 2478880..2479377 - 498 WP_000006256.1 REP-associated tyrosine transposase RayT -
C1P56_RS13070 (2479553) 2479553..2480326 - 774 WP_000543886.1 C40 family peptidase -
C1P56_RS13075 (2480512) 2480512..2480772 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1P56_RS13080 (2480775) 2480775..2481053 + 279 WP_000615975.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1P56_RS13085 (2481209) 2481209..2481949 + 741 WP_001225684.1 peptidoglycan meso-diaminopimelic acid protein amidase -
C1P56_RS13090 (2481920) 2481920..2482686 - 767 Protein_2553 class II glutamine amidotransferase -
C1P56_RS13095 (2482892) 2482892..2483470 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10853.66 Da        Isoelectric Point: 10.0703

>T96377 WP_000615975.1 NZ_CP026836:2480775-2481053 [Shigella boydii]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINHTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T96377 NZ_CP026836:2480775-2481053 [Shigella boydii]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAAGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATCATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT96377 WP_000729704.1 NZ_CP026836:2480512-2480772 [Shigella boydii]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT96377 NZ_CP026836:2480512-2480772 [Shigella boydii]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References