Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 270586..271213 Replicon chromosome
Accession NZ_CP026836
Organism Shigella boydii strain ATCC 49812

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag C1P56_RS01380 Protein ID WP_024258354.1
Coordinates 270884..271213 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag C1P56_RS01375 Protein ID WP_000147081.1
Coordinates 270586..270894 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P56_RS01345 (265880) 265880..266548 + 669 Protein_264 DnaB-like helicase N-terminal domain-containing protein -
C1P56_RS01355 (268845) 268845..269612 + 768 Protein_266 DnaB-like helicase C-terminal domain-containing protein -
C1P56_RS01360 (269686) 269686..269964 + 279 WP_134796271.1 hypothetical protein -
C1P56_RS01365 (269961) 269961..270371 + 411 WP_000814621.1 recombination protein NinB -
C1P56_RS01370 (270368) 270368..270544 + 177 WP_001254220.1 NinE family protein -
C1P56_RS01375 (270586) 270586..270894 - 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
C1P56_RS01380 (270884) 270884..271213 - 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P56_RS01390 (271535) 271535..272212 + 678 WP_119172668.1 P22AR C-terminal domain-containing protein -
C1P56_RS01395 (272287) 272287..273009 + 723 WP_001004003.1 DNA-binding protein -
C1P56_RS01400 (273047) 273047..273556 + 510 WP_119172665.1 NUMOD4 motif-containing HNH endonuclease -
C1P56_RS28085 (273589) 273589..273810 + 222 WP_134796716.1 hypothetical protein -
C1P56_RS01415 (275072) 275072..275977 + 906 Protein_277 IS66-like element ISSfl3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 254832..288350 33518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T96361 WP_024258354.1 NZ_CP026836:c271213-270884 [Shigella boydii]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T96361 NZ_CP026836:c271213-270884 [Shigella boydii]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT96361 WP_000147081.1 NZ_CP026836:c270894-270586 [Shigella boydii]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT96361 NZ_CP026836:c270894-270586 [Shigella boydii]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References