Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3568572..3569299 Replicon chromosome
Accession NZ_CP026832
Organism Shigella dysenteriae strain ATCC 49346

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C5B81_RS18875 Protein ID WP_000550189.1
Coordinates 3568985..3569299 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C5B81_RS18870 Protein ID WP_000560263.1
Coordinates 3568572..3568988 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B81_RS18855 (3565173) 3565173..3566879 + 1707 Protein_3619 NADPH-dependent 2,4-dienoyl-CoA reductase -
C5B81_RS18865 (3568201) 3568201..3568527 + 327 Protein_3621 2,4-dienoyl-CoA reductase -
C5B81_RS18870 (3568572) 3568572..3568988 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C5B81_RS18875 (3568985) 3568985..3569299 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C5B81_RS18880 (3569584) 3569584..3570720 - 1137 WP_134801128.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C5B81_RS18885 (3570805) 3570805..3571308 + 504 WP_005043640.1 M48 family metallopeptidase -
C5B81_RS18890 (3571385) 3571385..3572068 + 684 WP_001183035.1 vancomycin high temperature exclusion protein -
C5B81_RS18895 (3572147) 3572147..3573133 + 987 WP_004986115.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T96324 WP_000550189.1 NZ_CP026832:c3569299-3568985 [Shigella dysenteriae]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T96324 NZ_CP026832:c3569299-3568985 [Shigella dysenteriae]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT96324 WP_000560263.1 NZ_CP026832:c3568988-3568572 [Shigella dysenteriae]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT96324 NZ_CP026832:c3568988-3568572 [Shigella dysenteriae]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References