Detailed information of TA system TA096320

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2756798..2757023
• Replicon: chromosome
• Accession: NZ_CP026832
• Organism: Shigella dysenteriae strain ATCC 49346

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C5B81_RS14735
• Protein ID: WP_000813254.1
• Coordinates: 2756798..2756953 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2756965..2757023 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B81_RS14695	2751946..2753214	-	1269	Protein_2825	prophage tail fiber N-terminal domain-containing protein	-
C5B81_RS14700	2753238..2753783	-	546	WP_000902849.1	tail fiber assembly protein	-
C5B81_RS26805	2753786..2753854	-	69	Protein_2827	phage tail protein	-
C5B81_RS14705	2753910..2754607	+	698	WP_252988931.1	IS1-like element IS1SD family transposase	-
C5B81_RS14710	2754623..2755551	+	929	Protein_2829	IS3-like element IS600 family transposase	-
C5B81_RS14720	2755601..2756350	-	750	Protein_2830	hypothetical protein	-
C5B81_RS14725	2756352..2756630	-	279	WP_000929754.1	hypothetical protein	-
C5B81_RS14735	2756798..2756953	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2756965..2757023	+	59	-	-	Antitoxin
C5B81_RS14750	2757605..2758021	-	417	WP_005069274.1	hypothetical protein	-
C5B81_RS14755	2758133..2758513	+	381	WP_001333468.1	transposase	-
C5B81_RS14760	2758510..2758857	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C5B81_RS14765	2758906..2760444	+	1539	WP_000099181.1	IS66-like element ISEc22 family transposase	-
C5B81_RS14770	2760744..2761871	-	1128	WP_001063816.1	IS110-like element ISSso6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2726548..2804518	77970
	-	inside	IScluster/Tn	-	-	2754230..2768316	14086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T96320 WP_000813254.1 NZ_CP026832:c2756953-2756798 [Shigella dysenteriae]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T96320 NZ_CP026832:c2756953-2756798 [Shigella dysenteriae]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT96320 NZ_CP026832:2756965-2757023 [Shigella dysenteriae]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin