Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3841968..3842662 Replicon chromosome
Accession NZ_CP026831
Organism Shigella dysenteriae strain ATCC 12039

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag C1P71_RS21005 Protein ID WP_001521903.1
Coordinates 3841968..3842366 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag C1P71_RS21010 Protein ID WP_000554758.1
Coordinates 3842369..3842662 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P71_RS20975 (3838044) 3838044..3838565 - 522 WP_001143513.1 metal-dependent hydrolase -
C1P71_RS20980 (3838673) 3838673..3838885 - 213 WP_000190288.1 ribosome-associated protein YbcJ -
C1P71_RS20985 (3838887) 3838887..3839753 - 867 WP_000729161.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
C1P71_RS20990 (3840224) 3840224..3840556 + 333 Protein_3830 type 1 fimbrial major subunit FimA -
C1P71_RS21000 (3841506) 3841506..3841958 - 453 WP_071599453.1 GNAT family N-acetyltransferase -
C1P71_RS21005 (3841968) 3841968..3842366 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P71_RS21010 (3842369) 3842369..3842662 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P71_RS21015 (3842714) 3842714..3843769 - 1056 WP_001226177.1 DNA polymerase IV -
C1P71_RS21020 (3843840) 3843840..3844625 - 786 WP_000207554.1 putative lateral flagellar export/assembly protein LafU -
C1P71_RS21025 (3844597) 3844597..3846309 + 1713 Protein_3837 flagellar biosynthesis protein FlhA -
C1P71_RS21030 (3846471) 3846471..3847221 - 751 Protein_3838 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3840569..3841084 515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T96304 WP_001521903.1 NZ_CP026831:c3842366-3841968 [Shigella dysenteriae]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T96304 NZ_CP026831:c3842366-3841968 [Shigella dysenteriae]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT96304 WP_000554758.1 NZ_CP026831:c3842662-3842369 [Shigella dysenteriae]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT96304 NZ_CP026831:c3842662-3842369 [Shigella dysenteriae]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References