Detailed information of TA system TA096293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 773870..774597
• Replicon: chromosome
• Accession: NZ_CP026831
• Organism: Shigella dysenteriae strain ATCC 12039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C1P71_RS05120
• Protein ID: WP_128880035.1
• Coordinates: 773870..774181 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C1P71_RS05125
• Protein ID: WP_000126294.1
• Coordinates: 774178..774597 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P71_RS05090 (769395)	769395..769937	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C1P71_RS05095 (769937)	769937..770704	+	768	WP_128880033.1	formate hydrogenlyase subunit HycG	-
C1P71_RS05100 (770701)	770701..771111	+	411	WP_001291919.1	formate hydrogenlyase assembly protein HycH	-
C1P71_RS05105 (771104)	771104..771574	+	471	WP_128880034.1	hydrogenase maturation peptidase HycI	-
C1P71_RS05110 (771617)	771617..772372	+	756	WP_128881592.1	hypothetical protein	-
C1P71_RS05115 (772581)	772581..773809	+	1229	WP_088895425.1	IS3-like element IS2 family transposase	-
C1P71_RS05120 (773870)	773870..774181	+	312	WP_128880035.1	type II toxin-antitoxin system HigB family toxin	Toxin
C1P71_RS05125 (774178)	774178..774597	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C1P71_RS05130 (774676)	774676..776100	-	1425	WP_128880036.1	6-phospho-beta-glucosidase AscB	-
C1P71_RS05135 (776109)	776109..777557	-	1449	WP_128880037.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C1P71_RS05140 (777817)	777817..778827	+	1011	WP_171766313.1	DNA-binding transcriptional regulator AscG	-
C1P71_RS05145 (778976)	778976..779503	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12448.15 Da
• Isoelectric Point: 9.2371

>T96293 WP_128880035.1 NZ_CP026831:773870-774181 [Shigella dysenteriae]
MHIISKAPFEECAHKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T96293 NZ_CP026831:773870-774181 [Shigella dysenteriae]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCACAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT96293 WP_000126294.1 NZ_CP026831:774178-774597 [Shigella dysenteriae]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT96293 NZ_CP026831:774178-774597 [Shigella dysenteriae]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTTGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA