Detailed information of TA system TA096268

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2654391..2654616
• Replicon: chromosome
• Accession: NZ_CP026828
• Organism: Shigella dysenteriae strain ATCC 12037

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C1P70_RS13640
• Protein ID: WP_000813254.1
• Coordinates: 2654461..2654616 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2654391..2654449 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P70_RS13590	2649398..2649703	+	306	WP_001266147.1	DUF4406 domain-containing protein	-
C1P70_RS13595	2649690..2650145	+	456	WP_000335375.1	ead/Ea22-like family protein	-
C1P70_RS13600	2650195..2651732	-	1538	Protein_2608	IS66-like element ISEc22 family transposase	-
C1P70_RS13605	2651781..2652128	-	348	WP_000612610.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P70_RS13610	2652125..2652505	-	381	WP_001333468.1	transposase	-
C1P70_RS13615	2652617..2652865	+	249	Protein_2611	hypothetical protein	-
C1P70_RS13625	2653622..2653810	+	189	WP_134807086.1	hypothetical protein	-
-	2654391..2654449	-	59	-	-	Antitoxin
C1P70_RS13640	2654461..2654616	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
C1P70_RS13650	2654784..2655062	+	279	WP_000929754.1	hypothetical protein	-
C1P70_RS13655	2655064..2655813	+	750	Protein_2616	hypothetical protein	-
C1P70_RS13670	2657083..2657325	-	243	Protein_2618	response regulator	-
C1P70_RS13675	2657458..2657871	+	414	WP_000920127.1	hydroxyisourate hydrolase	-
C1P70_RS13680	2657980..2658980	+	1001	Protein_2620	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
C1P70_RS13685	2658981..2659616	+	636	WP_001241113.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2610983..2692336	81353
	-	inside	IScluster/Tn	-	-	2645311..2668929	23618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T96268 WP_000813254.1 NZ_CP026828:2654461-2654616 [Shigella dysenteriae]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T96268 NZ_CP026828:2654461-2654616 [Shigella dysenteriae]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT96268 NZ_CP026828:c2654449-2654391 [Shigella dysenteriae]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin