Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4455536..4456263 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C5B75_RS24545 Protein ID WP_000550189.1
Coordinates 4455536..4455850 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C5B75_RS24550 Protein ID WP_000560266.1
Coordinates 4455847..4456263 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS24525 (4451693) 4451693..4452679 - 987 WP_001297165.1 Gfo/Idh/MocA family oxidoreductase -
C5B75_RS24530 (4452758) 4452758..4453450 - 693 WP_000942535.1 vancomycin high temperature exclusion protein -
C5B75_RS24535 (4453527) 4453527..4454030 - 504 WP_001295542.1 M48 family metallopeptidase -
C5B75_RS24540 (4454115) 4454115..4455251 + 1137 WP_119170270.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C5B75_RS24545 (4455536) 4455536..4455850 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C5B75_RS24550 (4455847) 4455847..4456263 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C5B75_RS24555 (4456308) 4456308..4458326 - 2019 WP_119170268.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C5B75_RS26355 (4458652) 4458652..4458780 - 129 Protein_4499 hypothetical protein -
C5B75_RS24565 (4458835) 4458835..4459532 + 698 WP_094106546.1 IS1 family transposase -
C5B75_RS24570 (4459548) 4459548..4459751 - 204 Protein_4501 YhcH/YjgK/YiaL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4459029..4459532 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T96258 WP_000550189.1 NZ_CP026827:4455536-4455850 [Shigella dysenteriae]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T96258 NZ_CP026827:4455536-4455850 [Shigella dysenteriae]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT96258 WP_000560266.1 NZ_CP026827:4455847-4456263 [Shigella dysenteriae]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT96258 NZ_CP026827:4455847-4456263 [Shigella dysenteriae]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATATACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCAAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References