Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2708113..2708654 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag C5B75_RS15675 Protein ID WP_000615979.1
Coordinates 2708376..2708654 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C5B75_RS15670 Protein ID WP_000729704.1
Coordinates 2708113..2708373 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS15635 (2703237) 2703237..2704145 - 909 Protein_2769 3-(3-hydroxy-phenyl)propionate transporter MhpT -
C5B75_RS15640 (2704231) 2704231..2705421 + 1191 WP_128875526.1 IS4-like element ISEc60 family transposase -
C5B75_RS15650 (2705693) 2705693..2705971 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C5B75_RS15655 (2705964) 2705964..2706320 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C5B75_RS15660 (2706377) 2706377..2707150 - 774 Protein_2773 C40 family peptidase -
C5B75_RS26100 (2707846) 2707846..2708034 + 189 WP_252988466.1 hypothetical protein -
C5B75_RS15670 (2708113) 2708113..2708373 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C5B75_RS15675 (2708376) 2708376..2708654 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C5B75_RS15680 (2708810) 2708810..2709550 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
C5B75_RS15685 (2709521) 2709521..2710288 - 768 WP_001365365.1 class II glutamine amidotransferase -
C5B75_RS15690 (2710494) 2710494..2711072 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T96251 WP_000615979.1 NZ_CP026827:2708376-2708654 [Shigella dysenteriae]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T96251 NZ_CP026827:2708376-2708654 [Shigella dysenteriae]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT96251 WP_000729704.1 NZ_CP026827:2708113-2708373 [Shigella dysenteriae]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT96251 NZ_CP026827:2708113-2708373 [Shigella dysenteriae]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References