Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 995000..995502 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C5B75_RS06640 Protein ID WP_000767829.1
Coordinates 995248..995502 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C5B75_RS06635 Protein ID WP_001259255.1
Coordinates 995000..995251 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS06610 (990178) 990178..991245 - 1068 WP_000080118.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C5B75_RS06615 (991245) 991245..992315 - 1071 WP_000108954.1 histidinol-phosphate transaminase -
C5B75_RS06620 (992312) 992312..993616 - 1305 WP_119170482.1 histidinol dehydrogenase -
C5B75_RS06625 (993622) 993622..994521 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C5B75_RS06630 (994667) 994667..994717 - 51 WP_001364200.1 his operon leader peptide -
C5B75_RS06635 (995000) 995000..995251 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C5B75_RS06640 (995248) 995248..995502 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C5B75_RS06645 (995585) 995585..996409 + 825 WP_000754737.1 SDR family oxidoreductase -
C5B75_RS06650 (996455) 996455..997375 + 921 WP_128875090.1 LysR substrate-binding domain-containing protein -
C5B75_RS06655 (997404) 997404..998101 + 698 WP_094106546.1 IS1 family transposase -
C5B75_RS06660 (998376) 998376..998438 + 63 WP_010723108.1 membrane protein YoeI -
C5B75_RS06665 (998428) 998428..999786 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T96239 WP_000767829.1 NZ_CP026827:995248-995502 [Shigella dysenteriae]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T96239 NZ_CP026827:995248-995502 [Shigella dysenteriae]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT96239 WP_001259255.1 NZ_CP026827:995000-995251 [Shigella dysenteriae]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96239 NZ_CP026827:995000-995251 [Shigella dysenteriae]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References