Detailed information of TA system TA096237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 237944..238671
• Replicon: chromosome
• Accession: NZ_CP026827
• Organism: Shigella dysenteriae strain ATCC 12021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C5B75_RS02720
• Protein ID: WP_119169780.1
• Coordinates: 237944..238255 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5B75_RS02725
• Protein ID: WP_000126294.1
• Coordinates: 238252..238671 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B75_RS02690 (233086)	233086..234795	+	1710	WP_119169781.1	formate hydrogenlyase subunit HycE	-
C5B75_RS02695 (234805)	234805..235347	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
C5B75_RS02700 (235347)	235347..236114	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
C5B75_RS02705 (236111)	236111..236521	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C5B75_RS02710 (236514)	236514..236984	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C5B75_RS02715 (237009)	237009..237767	+	759	Protein_229	hypothetical protein	-
C5B75_RS02720 (237944)	237944..238255	+	312	WP_119169780.1	type II toxin-antitoxin system HigB family toxin	Toxin
C5B75_RS02725 (238252)	238252..238671	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C5B75_RS02730 (238786)	238786..240210	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
C5B75_RS02735 (240219)	240219..241676	-	1458	WP_128875006.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C5B75_RS02740 (241936)	241936..242946	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
C5B75_RS02745 (243095)	243095..243622	+	528	WP_119169779.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T96237 WP_119169780.1 NZ_CP026827:237944-238255 [Shigella dysenteriae]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLKVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T96237 NZ_CP026827:237944-238255 [Shigella dysenteriae]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAAGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT96237 WP_000126294.1 NZ_CP026827:238252-238671 [Shigella dysenteriae]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT96237 NZ_CP026827:238252-238671 [Shigella dysenteriae]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA