Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3606005..3606507 Replicon chromosome
Accession NZ_CP026814
Organism Shigella boydii strain 83-578

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C1P60_RS19590 Protein ID WP_000767829.1
Coordinates 3606253..3606507 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C1P60_RS19585 Protein ID WP_001259255.1
Coordinates 3606005..3606256 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P60_RS19560 3601183..3602250 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C1P60_RS19565 3602250..3603320 - 1071 WP_039080504.1 histidinol-phosphate transaminase -
C1P60_RS19570 3603317..3604621 - 1305 WP_039080505.1 histidinol dehydrogenase -
C1P60_RS19575 3604627..3605526 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C1P60_RS19580 3605672..3605722 - 51 WP_001364200.1 his operon leader peptide -
C1P60_RS19585 3606005..3606256 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C1P60_RS19590 3606253..3606507 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C1P60_RS19595 3606590..3607414 + 825 WP_000754747.1 SDR family oxidoreductase -
C1P60_RS19600 3607460..3608389 + 930 WP_039059232.1 LysR family transcriptional regulator -
C1P60_RS19605 3608604..3608666 + 63 WP_010723108.1 membrane protein YoeI -
C1P60_RS19610 3608656..3610014 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C1P60_RS19615 3610193..3611153 + 961 Protein_3544 YeeE/YedE family protein -
C1P60_RS19620 3611167..3611394 + 228 WP_000985274.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T96160 WP_000767829.1 NZ_CP026814:3606253-3606507 [Shigella boydii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T96160 NZ_CP026814:3606253-3606507 [Shigella boydii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT96160 WP_001259255.1 NZ_CP026814:3606005-3606256 [Shigella boydii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96160 NZ_CP026814:3606005-3606256 [Shigella boydii]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCTAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References