Detailed information of TA system TA096157

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3421402..3421627
• Replicon: chromosome
• Accession: NZ_CP026814
• Organism: Shigella boydii strain 83-578

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C1P60_RS18530
• Protein ID: WP_000813254.1
• Coordinates: 3421402..3421557 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3421569..3421627 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P60_RS18495	3416570..3418107	-	1538	Protein_3331	IS66-like element ISEc22 family transposase	-
C1P60_RS18500	3418156..3418503	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P60_RS18505	3418500..3418880	-	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
C1P60_RS18510	3419235..3420362	-	1128	WP_001063816.1	IS110-like element ISSso6 family transposase	-
C1P60_RS18515	3420493..3420954	-	462	Protein_3335	hypothetical protein	-
C1P60_RS18520	3420956..3421234	-	279	WP_000929754.1	hypothetical protein	-
C1P60_RS18530	3421402..3421557	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3421569..3421627	+	59	-	-	Antitoxin
C1P60_RS18545	3422209..3422651	-	443	Protein_3338	hypothetical protein	-
C1P60_RS18550	3422695..3423075	+	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
C1P60_RS18555	3423072..3423419	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P60_RS18560	3423468..3425006	+	1539	WP_134802545.1	IS66-like element ISEc22 family transposase	-
C1P60_RS18565	3425003..3425362	+	360	WP_073692993.1	3'-5' exoribonuclease	-
C1P60_RS18570	3425421..3425624	+	204	WP_000096344.1	DUF4224 domain-containing protein	-
C1P60_RS18575	3425624..3425979	+	356	Protein_3344	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	3412499..3440274	27775
	-	inside	IScluster/Tn	-	-	3414206..3427180	12974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T96157 WP_000813254.1 NZ_CP026814:c3421557-3421402 [Shigella boydii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T96157 NZ_CP026814:c3421557-3421402 [Shigella boydii]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT96157 NZ_CP026814:3421569-3421627 [Shigella boydii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin