Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1879851..1880578 Replicon chromosome
Accession NZ_CP026805
Organism Shigella dysenteriae strain 2017C-4522

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C1P73_RS10300 Protein ID WP_000550189.1
Coordinates 1880264..1880578 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C1P73_RS10295 Protein ID WP_000560263.1
Coordinates 1879851..1880267 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P73_RS25535 (1875211) 1875211..1877562 + 2352 Protein_1982 alpha-glucosidase -
C1P73_RS10290 (1877788) 1877788..1879806 + 2019 WP_000121440.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C1P73_RS10295 (1879851) 1879851..1880267 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C1P73_RS10300 (1880264) 1880264..1880578 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C1P73_RS10305 (1880863) 1880863..1881999 - 1137 WP_000018665.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C1P73_RS10310 (1882084) 1882084..1882587 + 504 WP_001295542.1 M48 family metallopeptidase -
C1P73_RS10315 (1882664) 1882664..1883347 + 684 WP_001183035.1 vancomycin high temperature exclusion protein -
C1P73_RS10320 (1883426) 1883426..1884412 + 987 WP_004986115.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T96091 WP_000550189.1 NZ_CP026805:c1880578-1880264 [Shigella dysenteriae]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T96091 NZ_CP026805:c1880578-1880264 [Shigella dysenteriae]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT96091 WP_000560263.1 NZ_CP026805:c1880267-1879851 [Shigella dysenteriae]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT96091 NZ_CP026805:c1880267-1879851 [Shigella dysenteriae]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References