Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3043167..3043861 Replicon chromosome
Accession NZ_CP026803
Organism Shigella flexneri strain 89-141

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P88_RS16085 Protein ID WP_001263491.1
Coordinates 3043167..3043565 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P88_RS16090 Protein ID WP_000554759.1
Coordinates 3043568..3043861 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P88_RS16060 (3038506) 3038506..3038964 - 459 WP_001291993.1 xanthine phosphoribosyltransferase -
C1P88_RS16065 (3039226) 3039226..3040683 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
C1P88_RS16070 (3040740) 3040740..3041300 - 561 Protein_3076 peptide chain release factor H -
C1P88_RS16075 (3041416) 3041416..3042459 - 1044 WP_005072768.1 RNA ligase RtcB family protein -
C1P88_RS16080 (3042705) 3042705..3043157 - 453 WP_001059864.1 GNAT family N-acetyltransferase -
C1P88_RS16085 (3043167) 3043167..3043565 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P88_RS16090 (3043568) 3043568..3043861 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P88_RS16095 (3043913) 3043913..3044968 - 1056 WP_001226172.1 DNA polymerase IV -
C1P88_RS16100 (3045039) 3045039..3045824 - 786 WP_000207553.1 putative lateral flagellar export/assembly protein LafU -
C1P88_RS16105 (3045796) 3045796..3047508 + 1713 Protein_3083 flagellar biosynthesis protein FlhA -
C1P88_RS16110 (3047725) 3047725..3048222 - 498 WP_000006243.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3019877..3076227 56350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T96070 WP_001263491.1 NZ_CP026803:c3043565-3043167 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T96070 NZ_CP026803:c3043565-3043167 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT96070 WP_000554759.1 NZ_CP026803:c3043861-3043568 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT96070 NZ_CP026803:c3043861-3043568 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References