Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1004871..1005498 Replicon chromosome
Accession NZ_CP026803
Organism Shigella flexneri strain 89-141

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag C1P88_RS05025 Protein ID WP_024258354.1
Coordinates 1004871..1005200 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag C1P88_RS05030 Protein ID WP_000147081.1
Coordinates 1005190..1005498 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P88_RS04995 (1000164) 1000164..1000500 + 337 Protein_958 DUF1175 family protein -
C1P88_RS05000 (1000513) 1000513..1001859 - 1347 WP_005076505.1 IS4-like element IS4 family transposase -
C1P88_RS05005 (1001930) 1001930..1002121 - 192 Protein_960 site-specific integrase -
C1P88_RS05010 (1002196) 1002196..1003364 + 1169 WP_000747032.1 IS3-like element IS911 family transposase -
C1P88_RS05015 (1003372) 1003372..1003791 - 420 Protein_962 Rha family transcriptional regulator -
C1P88_RS05020 (1003866) 1003866..1004552 - 687 WP_000218236.1 P22AR C-terminal domain-containing protein -
C1P88_RS05025 (1004871) 1004871..1005200 + 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P88_RS05030 (1005190) 1005190..1005498 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
C1P88_RS05035 (1005540) 1005540..1005716 - 177 WP_001254255.1 NinE family protein -
C1P88_RS05040 (1005713) 1005713..1006159 - 447 WP_000811308.1 recombination protein NinB -
C1P88_RS05045 (1006116) 1006116..1006412 - 297 WP_000049341.1 hypothetical protein -
C1P88_RS25270 (1006423) 1006423..1006518 - 96 Protein_969 hypothetical protein -
C1P88_RS25275 (1007853) 1007853..1007966 + 114 Protein_971 DUF4376 domain-containing protein -
C1P88_RS05065 (1008146) 1008146..1009909 - 1764 WP_000801167.1 NEL-type E3 ubiquitin ligase domain-containing protein -
C1P88_RS05070 (1010001) 1010001..1010087 + 87 Protein_973 transposase -
C1P88_RS05075 (1010097) 1010097..1010177 - 81 Protein_974 hypothetical protein -
C1P88_RS05080 (1010388) 1010388..1010477 + 90 Protein_975 DUF1175 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH4.5 975751..1018129 42378
- inside IScluster/Tn - ipaH4.5 1000513..1013423 12910


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T96057 WP_024258354.1 NZ_CP026803:1004871-1005200 [Shigella flexneri]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T96057 NZ_CP026803:1004871-1005200 [Shigella flexneri]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACCCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT96057 WP_000147081.1 NZ_CP026803:1005190-1005498 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT96057 NZ_CP026803:1005190-1005498 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References